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The 1 μL sample volume with 5 μL AuNPs’ conjugated mAbs and 54 μL running buffer calibration curves in ( a ) OD values’ optimisation in regard to OD 10, OD 5, OD 2.5; ( b ) calibration curves with CN 95, CN 110, and CN140 (OD 5 AuNPs).

Journal: Biosensors

Article Title: Performance of Colorimetric Lateral Flow Immunoassays for Renal Function Evaluation with Human Serum Cystatin C

doi: 10.3390/bios15070445

Figure Lengend Snippet: The 1 μL sample volume with 5 μL AuNPs’ conjugated mAbs and 54 μL running buffer calibration curves in ( a ) OD values’ optimisation in regard to OD 10, OD 5, OD 2.5; ( b ) calibration curves with CN 95, CN 110, and CN140 (OD 5 AuNPs).

Article Snippet: A λ is the absorbance at the wavelength λ [ ]. (1) O D λ = A λ L = 1 L log 10 I 0 I In total, 40 OD AuNPs (40 nm, ab269942, Abcam, Cambridge, UK) were conjugated with the 89.5 μg/mL detector mAbs (Fitzgerald, 10-7887, Stratech, Ely, UK).

Techniques: